Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of expression profiles of once and three time linearly amplified samples

ABSTRACT: In order to assess the impact of three rounds of linear amplification on the technical reproducibility of gene expression measurements, we performed twelve microarray experiments. We analysed mouse RNA from cortex, cerebellum and liver from one individual. One RNA sample of 5µg from each of the three different tissues was processed according to the standard Affymetrix protocol and hybridized onto mouse gene expression arrays MG_U74Av2. Three additional samples from each tissue of 1ng were processed according to a modified procedure that involves three linear amplifications before hybridization onto the microarray chips. One mouse was analysed. One RNA sample of 5µg from each of liver, cortex, and cerebellum were processed according to the standard Affymetrix protocol and hybridized onto mouse gene expression arrays MG_U74Av2. Three additional samples (technical replicates) from each tissue of 1ng were processed according to a modified procedure that involves three linear amplifications before hybridization onto the microarray chips, resulting in 12 microarray experiments.

ORGANISM(S): Mus musculus

SUBMITTER: Thomas Giger 

PROVIDER: E-GEOD-21389 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The study of gene expression evolution in vertebrates has hitherto focused on the analysis of transcriptomes in tissues of different species. However, because a tissue is made up of different cell types, and cell types differ with respect to their transcriptomes, the analysis of tissues offers a composite picture of transcriptome evolution. The isolation of individual cells from tissue sections opens up the opportunity to study gene expression evolution at the cell type level. We have stained ne  ...[more]

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