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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of genes that regulated by IRF4 in human cutaneous T cell lymphoma HuT-102 cells

ABSTRACT: The microarray analysis showed an interesting up-regulation in the set of genes controlling the development of Th1, mainly IFN-gamma, and other type 1 interferon response genes including CXCL10 in IRF4 knocked-down HuT-102 cells. HuT-102 cells, a human cutaneous T cell lymphoma, were transiently transfected with siRNAs against human IRF4 or firefly luciferase. Cells were incubated for 60 h at 37 centigrade. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâs instructions.

ORGANISM(S): Homo sapiens

SUBMITTER: Yoshiaki Tabuchi

PROVIDER: E-GEOD-22036 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes that are regulated by TAK1 in human cutaneous T cell lymphoma HuT-102 cells

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