Experimental annotation of the human pathogen Candida albicans coding and noncoding transcribed regions using high resolution tiling arrays

ABSTRACT: Compared to other model organisms and despite the clinical relevance of the pathogenic yeast Candida albicans, no comprehensive analysis has been done to provide experimental support of its in silico-based genome annotation. Here we have undertaken a genome-wide experimental annotation to accurately uncover the transcriptional landscape of the pathogenic yeast C. albicans using strand-specific high-density tiling arrays. RNAs were purified from cells growing under conditions relevant to C. albicans pathogenicity, including biofilm, lab-grown yeast and serum-induced hyphae as well as cells isolated from the mouse caecum. This work provides a genome-wide experimental validation for a large number of predicted ORFs for which transcription had not been detected by other approaches. Additionall

ORGANISM(S): Candida albicans

SUBMITTER: Adnane Sellam 

PROVIDER: E-GEOD-22625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress