Transciptomic analysis of the hal4hal5 mutant of Saccharomyces cerevisiae

ABSTRACT: The yeast protein kinases Sat4/Hal4 and Hal5 are required for the plasma membrane stability of the K+ transporter Trk1 and some amino acid and glucose permeases. The transcriptomic analysis presented here indicates alterations in the general control of both nitrogen and carbon metabolism. Accordingly, we observed reduced uptake of methionine and leucine in the hal4 hal5 mutant. This decrease correlates with activation of the Gcn2-Gcn4 pathway, as measured by expression of the lacZ gene under the control of the Gcn4 promoter. However, with the exception of methionine biosynthetic genes, few amino acid biosynthetic genes are induced in the hal4 hal5 mutant, whereas several genes involved in amino acid catabolism are repressed. Concerning glucose metabolism, we found that this mutant exhibits

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jorge Pérez-Valle 

PROVIDER: E-GEOD-22976 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress