Severe alkaline pH stress induces a glucose starvation-related transcriptional response in S. cerevisiae

ABSTRACT: Alkaline pH stress invokes in S. cerevisiae a potent and fast transcriptional response that includes many genes repressed by glucose. Certain mutants in the glucose-sensing and response pathways, such as those lacking the Snf1 kinase, are sensitive to alkalinization. We show that addition of glucose to the medium improves growth of wild type cells at high pH, fully abolish the snf1 alkali-sensitive phenotype and attenuates high pH-induced Snf1 phosphorylation at Thr210. The elm1 mutant, lacking one of the three upstream Snf1 kinases (tos3, elm1 and sak1), is markedly alkali sensitive, whereas the phenotype of the tos3 elm1 sak1 strain is even stronger than that of snf1 cells and it is not fully rescued by glucose supplementation. DNA microarray analysis reveals that about 75% of genes indu

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Antonio Casamayor 

PROVIDER: E-GEOD-25697 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress