Subtractive cloning of differentially expressed genes in chick heart/hemangioblast precursor cells

ABSTRACT: Genetic evidence has implicated several genes as being critical for heart development. However, the inducers of these genes as well as their other targets and the pathways they constitute, remain largely unknown. In the avian embryo, Cerberus (cCer) transcripts are detected in the anterior mesendoderm including the heart precursor cells and in the left lateral plate mesoderm. We have identified a promoter element of chick cCer able to drive EGFP expression into the heart and the hemangioblast precursor cells, allowing us to identify a population of cells that consistently exit from the anterior primitive streak region from as early as stage HH3+ and that later will populate the heart. In order to identify and study novel genes expressed and involved in the correct development and differ

ORGANISM(S): Gallus gallus

SUBMITTER: Margaret Bento 

PROVIDER: E-GEOD-23389 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress