Degeneration of penicillin production in ethanol-limited chemostat cultivation of Penicillium chrysogenum: A systems biology approach

ABSTRACT: In microbial production of non-catabolic products, a loss of production capacity upon long-term cultivation (for example, chemostat), a phenomenon called strain degeneration, is nearly always observed. In this study, a systems biology approach (monitoring changes from gene to produced flux) was used to study degeneration of penicillin production by Penicillium chrysogenum in ethanol-limited chemostat fermentations where the biomass specific penicillin production rate decreased 10-fold within 30 generations. Results showed that the copy number of penicillin gene clusters and expression levels of central metabolism showed little decrease. With respect to penicillin production, major changes were observed: a strong downregulation of the cysteine pathway in agreement with its nearly 10-fold fl

ORGANISM(S): Penicillium chrysogenum

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-24212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress