Impact of Velvet complex on transcriptome and penicillin G production in glucose-limited chemostat cultures of a beta-lactam high-producing Penicillium chrysogenum strain

ABSTRACT: The multi-component global regulator Velvet complex has been identified as a key regulator of secondary metabolite production in Aspergillus and Penicillium species. Previous work indicated a massive impact of PcvelA and PclaeA deletions, two key components of the Velvet complex, on penicillin production in prolonged batch cultures of P. chrysogenum, as well as substantial changes in transcriptome. The present study investigates the impact of these mutations on product formation and genome-wide transcript profiles under glucose-limited, aerobic conditions, relevant for industrial production of ?-lactams. The gene-deletion cassette for PcvelA or PclaeA was integrated in a hdfA mutant of the penicillin high-producing strain P. chrysogenum DS17690. Predicted amino acid sequences of PcVelA and

ORGANISM(S): Penicillium chrysogenum

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-31633 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress