Genome-wide profiling of H3K56 acetylation and transcription factor binding sites in human adipocytes

ABSTRACT: We use ChIP-seq to discover the genome-wide sites of acetylation of lysine 56 of the histone H3 (H3K56), which is a target of three histone modifying enzymes with known roles in diabetes and insulin resistance, in human adipocytes derived from mesenchymal stem cells. Surprisingly, we find that a very large fraction of genes show some level of acetylation on H3K56, but the highest levels of acetylation are associated with genes previously reported to be involved in type 2 diabetes. Using computational methods, we propose that the transcription factor E2F4 may be involved in recruiting histone modifying enzymes to these sites. We confirm this prediction by measuring the binding of E2F4 using ChIP-seq. We also examine the binding of two other proteins using ChIP-Seq: HSF-1 and C/EBPM-NM-1

ORGANISM(S): Homo sapiens

SUBMITTER: Kinyui Alice LO 

PROVIDER: E-GEOD-24326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress