Comparison of gene expression of L. reuteri ATCC 55730 at different growth phase in LDMIII medium
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ABSTRACT: Comparison of gene expression between L. reuteri ATCC 55730 in LDMIII between early log phase (T8), late log phase (T12) or early stationary phase phase (T16) or late stationary phase (T24) Include 3 biological replicate and dye-swap for each comparison. Reference time point =T8
Project description:Comparison of gene expression between L. reuteri ATCC PTA 6475 in LDMIII between early log phase (T8), late log phase (T12) or early stationary phase phase (T16) or late stationary phase (T24) Include 3 biological replicate and dye-swap for each comparison. Reference time point =T8
Project description:This SuperSeries is composed of the following subset Series: GSE24415: Comparison of gene expression of L. reuteri ATCC PTA 6475 at different growth phase in a LDMIII GSE24570: Comparison of gene expression of L. reuteri ATCC 55730 at different growth phase in LDMIII medium Refer to individual Series
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 grown (stationary phase) in a defined media with either glucose or sucrose as the carbon source, in anaerobic conditions at 37C. Includes 2 biological replicates and dye-swaps for each comparison. Reference time point is is L. reuteri ATCC PTA 6475 grown in a glucose-based media.
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 and cyclopropane-fatty-acyl-phospholipid synthase mutant in MRS medium in anaerobic condition at 37C Include 3 biological replicate and dye-swap for each comparison. Reference time point = L. reuteri ATCC PTA 6475
Project description:Comparison of gene expression between L. reuteri ATCC PTA 6475 and PocR mutant grown in semi-defined medium after 24h of growth at 37C in anaerobic condition Include 3 biological replicate and dye-swap
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 and tetrahydrofolate synthase 1 in MRS medium in anaerobic conditions at 37C. Includes 3 biological replicates and dye-swaps, with one additional replicate for ATCC PTA 6475 versus tetrahydrofolate synthase 1 mutant. Reference time point is L. reuteri ATCC PTA 6475.
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 and tetrahydrofolate synthase 2 mutant in MRS medium in anaerobic conditions at 37C. Includes 3 biological replicates and dye-swaps for ATCC PTA 6475 versus tetrahydrofolate synthase 2 mutant. Reference time point is L. reuteri ATCC PTA 6475.
Project description:We used Chlamydomonas microarray v2.0 to compare the time course expression profiles of two Chlamydomonas reinhardtii strains: wild-type WT and the high hydrogen producing mutant Stm6Glc4 during sulfur starvation induced hydrogen production. Major cellular reorganizations in photosynthetic apparatus, sulfur and carbon metabolism upon H2 production were confirmed as common to both strains. More importantly, our results pointed out factors which lead to the higher hydrogen production in the mutant including higher light sensitivity and lower competitions with hydrogenase by alternative electron sinks. Under S-starvation induced H2 producing conditions the induction of LHCSR3, a chlorophyll binding protein involving in non photochemical quenching, was significantly lower in Stm6Glc4 resulting in significant higher photodamage to photosystem II. Consequently, Stm6Glc4 had a shorter aerobic phase, consumed less starch reserves, and produced H2 earlier at higher rates than WT. We also showed that the loss of mitochondrial DNA-binding protein MOC1 in both knockdown and knockout mutant resulted in higher light sensitivity and improved H2 yield. Furthermore, by comparing our data with previously published ‘omics’ data, we were able to identify genes that responded specifically to either sulfur starvation, anaerobiosis or hydrogen production as well as to provide a more complete picture of S-deprived H2 production in the green alga C. reinhardtii. A total of 33 microarray hydridizations were performed covering samples taken during the course of S deprivation induced H2 producction. The samples included 4 time points in the high hydrogen producing mutant Stm6Glc4 (taken at 16, 28, 52 and 76h) and 6 time points in the wildtype CC-406 (taken at 16, 28, 52, 68, 92, 116h). Samples from each time point were compared directly with the sample taken prior to S starvation from the corresponding strain. Three biological replicates were tested at each time point.
Project description:Bi-sex male and female Schistosoma mansoni worms were isolated from mice 7-weeks post-infection. Bi-sex male material or female material was compared in a direct bimodal comparison to single-sex male or female material. Two independent biological batches of both bi- and single-sex males were used. One batch of single-sex female material was used in comparisons to three independent biological batches of bi-sex female material. A dye-swap hybridization was performed for each bimodal comparisons in turn.
Project description:Transcriptional responses of two strain types of Mycobacterium avium subsp. paratuberculosis (MAP, cattle and sheep Strain) under in vitro iron limiting or iron sufficient growth conditions. Background: In MAP, the transcriptional role and essentiality of MAP2827 (IdeR) as an iron dependent regulator has been well established (Janagama et al. 2009). Therefore, in the absence of an ideR deletion mutant of MAP, to understand the genome-wide iron dependent transcriptional variations between the cattle and sheep MAP IdeR we used a heterologous expression of MAP IdeR in Mycobacterium smegmatis ideR deletion mutant.