Lactobacillus reuteri strains at different growth phases
Ontology highlight
ABSTRACT: This SuperSeries is composed of the following subset Series: GSE24415: Comparison of gene expression of L. reuteri ATCC PTA 6475 at different growth phase in a LDMIII GSE24570: Comparison of gene expression of L. reuteri ATCC 55730 at different growth phase in LDMIII medium Refer to individual Series
Project description:Comparison of gene expression between L. reuteri ATCC 55730 in LDMIII between early log phase (T8), late log phase (T12) or early stationary phase phase (T16) or late stationary phase (T24) Include 3 biological replicate and dye-swap for each comparison. Reference time point =T8
Project description:Comparison of gene expression between L. reuteri ATCC PTA 6475 in LDMIII between early log phase (T8), late log phase (T12) or early stationary phase phase (T16) or late stationary phase (T24) Include 3 biological replicate and dye-swap for each comparison. Reference time point =T8
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 and cyclopropane-fatty-acyl-phospholipid synthase mutant in MRS medium in anaerobic condition at 37C Include 3 biological replicate and dye-swap for each comparison. Reference time point = L. reuteri ATCC PTA 6475
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 and tetrahydrofolate synthase 2 mutant in MRS medium in anaerobic conditions at 37C. Includes 3 biological replicates and dye-swaps for ATCC PTA 6475 versus tetrahydrofolate synthase 2 mutant. Reference time point is L. reuteri ATCC PTA 6475.
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 and tetrahydrofolate synthase 1 in MRS medium in anaerobic conditions at 37C. Includes 3 biological replicates and dye-swaps, with one additional replicate for ATCC PTA 6475 versus tetrahydrofolate synthase 1 mutant. Reference time point is L. reuteri ATCC PTA 6475.
Project description:Gene expression comparison of L. reuteri ATCC PTA 6475 grown (stationary phase) in a defined media with either glucose or sucrose as the carbon source, in anaerobic conditions at 37C. Includes 2 biological replicates and dye-swaps for each comparison. Reference time point is is L. reuteri ATCC PTA 6475 grown in a glucose-based media.
Project description:Comparison of gene expression between L. reuteri ATCC PTA 6475 and PocR mutant grown in semi-defined medium after 24h of growth at 37C in anaerobic condition Include 3 biological replicate and dye-swap
Project description:Bi-sex male and female Schistosoma mansoni worms were isolated from mice 7-weeks post-infection. Bi-sex male material or female material was compared in a direct bimodal comparison to single-sex male or female material. Two independent biological batches of both bi- and single-sex males were used. One batch of single-sex female material was used in comparisons to three independent biological batches of bi-sex female material. A dye-swap hybridization was performed for each bimodal comparisons in turn.
Project description:Transcriptional responses of two strain types of Mycobacterium avium subsp. paratuberculosis (MAP, cattle and sheep Strain) under in vitro iron limiting or iron sufficient growth conditions. Background: In MAP, the transcriptional role and essentiality of MAP2827 (IdeR) as an iron dependent regulator has been well established (Janagama et al. 2009). Therefore, in the absence of an ideR deletion mutant of MAP, to understand the genome-wide iron dependent transcriptional variations between the cattle and sheep MAP IdeR we used a heterologous expression of MAP IdeR in Mycobacterium smegmatis ideR deletion mutant.
Project description:Transcriptional profiling of Lactobacillus reuteri ATCC 55730 mid-log cultures before vs after exposure to 0.5% bovine bile (oxgall). Two sets of array experiments were performed. One set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had been exposed to 0.5% bile for 15 minutes (bile shock). The other set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had begun growing again in the presence of 0.5% bile (bile adaptation). Two sets of two condition experiments: Set one: before bile treatment and 15 minutes after bile treatment. Biological replicates: 5 untreated and 5 treated, independently grown and collected. Two replicates per biological replicate (dye-swap). Set two: before bile treatment and after the resumption of growth in the presence of bile. Biological replicates: 5 untreated and 5 treated, independently grown and collected. Two replicates per biological replicate (dye-swap).