Potentiation of glucocorticoid signaling by chloroquine via inhibition of lysosomes

ABSTRACT: Anti-malaria drug chloroquine has been used as an anti-inflammatory agent for treating systemic lupus erythematosus and rheumatoid arthritis, without clear mechanism of action. Here we report that chloroquine potently inhibits the expression of proinflammatory cytokines through transrepression of glucocorticoid receptor (GR). Instead of direct binding to GR, chloroquine synergistically activates glucocorticoid signaling via inhibition of lysosomal functions. In mouse collagen induced arthritis model, chloroquine synergizes the therapeutic effects of glucocorticoid. Lysosomal inhibition by bafilomycin A1, an inhibitor of V-type ATPase, or by knockdown of transcription factor EB (TFEB), a master activator of lysosomal biogenesis, mimics the effects of chloroquine. These results reveal an

ORGANISM(S): Homo sapiens

SUBMITTER: Karl Dykema 

PROVIDER: E-GEOD-24579 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress