Hierarchical regulation in a KRAS-dependent transcriptional network revealed by a reverse-engineering approach

ABSTRACT: Mutations in RAS proteins occur in 30% of human tumours and have a high relevance in tumor progression. Despite the importance of the underlying genetic network that governs the effects of oncogenic RAS, it is still poorly understood. We developed and applied a reverse-engineering approach in order to reconstruct the network structure of the signaling and gene-regulatory network downstream of RAS from perturbation experiments. We performed microarray, RT-PCR and Western Blot analysis to detect mRNA and protein levels of cytoplasmatic and nuclear targets downstream of RAS after systematic perturbation of the signaling pathways and knock-down of selected transcription factors in KRAS-transformed ovarian surface epithelium cell lines. The reconstructed model shows that the investigated compon

ORGANISM(S): Rattus norvegicus

SUBMITTER: Oleg Tchernitsa 

PROVIDER: E-GEOD-24668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress