Geminin-regulated genes during neural fate acquisition of mouse embryonic stem cells

ABSTRACT: Formation of the complex vertebrate nervous system begins when pluripotent cells of the early embryo are directed to acquire a neural fate. Although cell intrinsic controls play an important role in this process, the molecular nature of this regulation is not well defined. Here we assessed the role for Geminin, a nuclear protein expressed in embryonic cells, in neural fate acquisition from mouse embryonic stem (ES) cells. While Geminin knockdown does not affect the ability of ES cells to maintain or exit pluripotency, we found that it significantly impairs their ability to acquire a neural fate. Conversely, Geminin overexpression promotes neural gene expression, even in the presence of growth factor signaling that antagonizes neural transcriptional responses. These data demonstrate that

ORGANISM(S): Mus musculus

SUBMITTER: Kristen Kroll 

PROVIDER: E-GEOD-25737 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress