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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (CGH)

ABSTRACT: Fetal mice (16 days gestation) were administered FIV-based lentiviral viral particles containing the gene encoding EGFP. Six liver tumors developed in three mice (1 male, 2 females) between the ages of 273 and 484 days, each mouse developed 2 tumors. These tumors and non-tumorous liver tissue from the same animals were harvested and aCGH was performed on genomic DNA extracted from these samples. We were interested in investigating the link between lentiviral integration and chromosomal aberrations. Two tumors from each mouse (1 male, 2 females) were each compared to non-tumorous tissue surrounding the tumors.

ORGANISM(S): Mus musculus

SUBMITTER: Reba Condiotti

PROVIDER: E-GEOD-25900 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transduction of fetal mice with a feline lentiviral vector induces liver tumors which exhibit an E2F activation signature.

Condiotti Reba R Goldenberg Daniel D Giladi Hilla H Schnitzer-Perlman Temima T Waddington Simon N SN Buckley Suzanne Mk SM Heim Denise D Cheung Wing W Themis Matthew M Coutelle Charles C Simerzin Alina A Osejindu Emma E Wege Henning H Themis Michael M Galun Eithan E

Molecular therapy : the journal of the American Society of Gene Therapy 20130828 1

Lentiviral vectors are widely used in basic research and clinical applications for gene transfer and long-term expression; however, safety issues have not yet been completely resolved. In this study, we characterized hepatocarcinomas that developed in mice 1 year after in utero administration of a feline-derived lentiviral vector. Mapped viral integration sites differed among tumors and did not coincide with the regions of chromosomal aberrations. Furthermore, gene expression profiling revealed ...[more]

PMID: 23982166

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Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (expression)

Project description:Fetal mice (16 days gestation) were administered feline immunodeficiency virus (FIV)-based lentiviral viral particles containing the gene encoding GFP. Six liver tumors developed in three mice between the ages of 273 and 484 days, each mouse developed 2 tumors. These tumors and non-tumorous liver tissue from the same animals and animals that did not develop tumors and untransduced controls were harvested and microarrays were performed on total RNA extracted from these samples. We were interested in investigating the link between lentiviral integration and gene expression. Keywords: Comparison of HCC with non-tumorous liver tissue adjacent to tumor. Fourteen samples were analyzed in total: Two tumors from each of three mice (6 samples); surrounding non-tumorous liver tissue from each of these three mice (3 samples); two female mice and one male mouse that were transduced and did not develop tumors (3 samples); one female and one male untransduced mouse (2 samples). The samples were performed in two runs: one containing the samples from female mice and the second containing samples from male mice.

2013-09-11 | E-GEOD-19306 | biostudies-arrayexpress

Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (CGH)

Project description:Fetal mice (16 days gestation) were administered FIV-based lentiviral viral particles containing the gene encoding EGFP. Six liver tumors developed in three mice (1 male, 2 females) between the ages of 273 and 484 days, each mouse developed 2 tumors. These tumors and non-tumorous liver tissue from the same animals were harvested and aCGH was performed on genomic DNA extracted from these samples. We were interested in investigating the link between lentiviral integration and chromosomal aberrations.

2013-09-11 | GSE25900 | GEO

Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (expression)

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High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development (aCGH)

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2012-10-31 | E-GEOD-31097 | biostudies-arrayexpress

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