Transcription profiling of human tetracycline-regulated cell line expressing an NF-kB inhibitor to systematically identify NF-kB dependent genes

ABSTRACT: TNF time course series of HelA tet off cells cultured in presence or absence of Dox; TNF is a pro-inflammatory cytokine that controls expression of inflammatory genetic networks. Although the Nuclear Factor-kB (NF-kB) pathway is crucial for mediating cellular TNF responses, the complete spectrum of NF-kB dependent genes is unknown. In this study, we used a tetracycline-regulated cell line expressing an NF-kB inhibitor to systematically identify NF-kB dependent genes. A microarray data set generated from a time course of TNF stimulation in the presence or absence of NF-kB signaling was analyzed. Methods: Cell culture, treatment and transfection-The human cervical epithelioid carcinoma cell line HeLa expressing tTA (HeLa Tet-Off) pBI-EGFP- IkBa Mut was constructed earlier (23). HeLa Tet-

ORGANISM(S): Homo sapiens

SUBMITTER: Allan Brasier 

PROVIDER: E-GEOD-2624 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress