Signaling to Transcription Networks in the Neuronal Retrograde Injury Response

ABSTRACT: Retrograde signaling from axon to soma activates intrinsic regeneration mechanisms in lesioned peripheral sensory neurons; however, the links between axonal injury signaling and the cell body response are not well understood. Here, we used phosphoproteomics and microarrays to implicate ~900 phosphoproteins in retrograde injury signaling in rat sciatic nerve axons in vivo and ~4500 transcripts in the in vivo response to injury in the dorsal root ganglia. Computational analyses of these data sets identified ~400 redundant axonal signaling networks connected to 39 transcription factors implicated in the sensory neuron response to axonal injury. Experimental perturbation of individual overrepresented signaling hub proteins, including Abl, AKT, p38, and protein kinase C, affected neurite outgro

ORGANISM(S): Rattus norvegicus

SUBMITTER: Giovanni Coppola 

PROVIDER: E-GEOD-26350 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress