MNase digestion-sensitive nucleosomes in Saccharomyces cerevisiae

ABSTRACT: The structural complexity of nucleosomes underlies their functional versatility. Here we report a new type of complexity – nucleosome fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumption that nucleosomes are similar in resistance to MNase digestion. Using differential MNase digestion of chromatin and high-throughput sequencing, we have identified a special group of nucleosomes termed fragile nucleosomes throughout the yeast genome, nearly one thousand of which are at previously determined “nucleosome free” loci. Nucleosome fragility is broadly implicated in multiple chromatin processes, including transcription, translocation and replication, in correspondence to specific physiological states of cells. In the environmental-stress-respons

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yuanxin Xi 

PROVIDER: E-GEOD-26412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress