Two Distinct Promoter Nucleosome Architectures at Protein-Coding Genes in Yeast

ABSTRACT: Previous studies indicate that eukaryotic promoters display a stereotypical chromatin landscape characterized by a well-positioned +1 nucleosome near the transcription start site and an upstream -1 nucleosome that together demarcate a nucleosome-free (or depleted) region. Here we present evidence that there are two distinct types of promoters distinguished by the resistance of the -1 nucleosome to micrococcal nuclease digestion. These different architectures are characterized by two sequence motifs that are broadly deployed at one set of promoters where a nuclease-sensitive ("fragile") nucleosome forms, but concentrated in a more narrow, nucleosome-free region at all other promoters. The RSC nucleosome remodeler acts through the motifs to establish stable +1 and -1 nucleosome pos

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jacques Rougemont 

PROVIDER: E-GEOD-73337 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress