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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromosome segregation in Streptococcus pneumoniae

ABSTRACT: Segregation of replicated chromosomes during cell division is an essential process in all organisms. Chromosome segregation is promoted by the action of the DNA-binding ParB protein in the rod-shaped model bacterium Bacillus subtilis. How oval shaped bacteria, such as the human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here, we show that the pneumococcal homolog of ParB is enriched at four centromere-like DNA sequences (parS sites) that are present near the origin of replication. Amplified ChIP DNA was fluorescently labelled using the BioPrime Total Genomic Labeling kit from Invitrogen. Eluate DNA was labelled with AlexA Fluor 3 and input DNA with Alexa Fluor 5. Labelled DNA was hybridized to a DNA-microarray containing amplicons of all open reading frames of S. pneumoniae (Kloosterman et al., 2006).

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Jan-Willem Veening

PROVIDER: E-GEOD-26839 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae.

Minnen Anita A Attaiech Laetitia L Thon Maria M Gruber Stephan S Veening Jan-Willem JW

Molecular microbiology 20110622 3

Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacil ...[more]

PMID: 21651626

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