Mouse embryonic and induced pluripotent stem cells can form definitive endoderm despite differences in imprinted genes

ABSTRACT: The directed differentiation of induced pluripotent stem (iPS) and embryonic stem (ES) cells into definitive endoderm (DE) would allow the derivation of otherwise inaccessible progenitors for endodermal tissues. However, a global comparison of the relative equivalency of DE derived from iPS and ES populations has not been performed. Recent reports of molecular differences between iPS and ES cells have raised uncertainty as to whether iPS cells could generate autologous endodermal lineages in vitro. Here, we have shown that both mouse iPS and parental ES cells exhibited highly similar in vitro capacity to undergo directed differentiation into DE progenitors. With few exceptions, both cell types displayed similar surges in gene expression of specific master transcriptional regulators and glo

ORGANISM(S): Mus musculus

SUBMITTER: steve shen 

PROVIDER: E-GEOD-27087 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress