Incomplete X-inactivation initiated by a hypomorphic Xist allele in the mouse

ABSTRACT: Mammals have evolved a mechanism to compensate for the difference in the number of X chromosome genes between XX females and XY males by inactivating one of the two X chromosomes in females. This X chromosome inactivation phenomenon is triggered by Xist gene, which is essential for X-inactivation to occur in cis. We demonstrate that an Xist mutant, XistIVS, is unique in that it retains a partial function to silence the X chromosome, which would provide an opportunity to dissect the molecular mechanism of Xist RNA-mediated chromosome silencing. To study the effect of XistIVS mutation on silencing of X-linked genes, global expression levels of X-linked genes were measured in mutant and control cells. We used female trophoblast stem (TS) cells carrying XistIVS mutation and gene expression was

ORGANISM(S): Mus musculus

SUBMITTER: Rieko Ikeda 

PROVIDER: E-GEOD-27373 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress