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Escape from X inactivation is directly modulated by Xist non-coding RNA [cell line RNA-seq]

ABSTRACT: X-chromosome inactivation silences one copy of the X-chromosome in female cells, but a number of genes escapes this inactivation. We asked whether increased levels of Xist RNA, the molecular actor driving X-inactivation, impacts expression levels of escapees. To this end, we performed transcriptomic profiling of X-linked gene expression in clonal neural progenitor cell lines with transgenes that allow for the overexpression of Xist on the inactive X chromosome. We perform Xist overexpression experiments for up to three weeks and also test the reversibility of Xist overexpression using washout experiments. Finally, we demonstrate the role of SPEN in Xist-mediated silencing of escape using an inducible degron.

ORGANISM(S): Mus musculus Mus

PROVIDER: GSE259399 | GEO | 2025/12/09

REPOSITORIES: GEO

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Escape from X inactivation is directly modulated by Xist non-coding RNA [methylation]

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