Transcription profilfing of human cerebrovascular smooth muscle to identify Ca2+-dependent transcription patterns

ABSTRACT: Altered Ca2+ handling has both immediate physiological effects and long-term genomic effects on vascular smooth muscle function. Previously we have shown that elevation of cytoplasmic Ca2+ through voltage-dependent Ca2+ channels (VDCCs) or store-operated Ca2+ channels (SOCCs) results in phosphorylation of the Ca2+/cAMP response element binding protein (CREB) in cerebral arteries. Here we demonstrate that stimulation of these different Ca2+ influx pathways results in transcriptional activation of a distinct, yet overlapping set of genes, and that the induction of selected CRE-regulated genes is prevented by the addition of corresponding Ca2+ channel blockers. Using oligonucleotide array analysis, changes in mRNA levels were quantified following membrane depolarization with K+ or depletio

ORGANISM(S): Homo sapiens

SUBMITTER: Anjanette Watson 

PROVIDER: E-GEOD-2883 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress