Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Numb variant specific transcriptome analysis

ABSTRACT: Numb has 4 known variants and we are reporting two novel Numb variants, Numb5 and Numb6 in this study. The metastaticability variations between the numb variants were evaluated through the transcriptome. breast cancer cells were transfected with GFP-Numb4, GFP-Numb5, GFP-Numb6 or GFP-only vectors and their transcriptome were assessed on Affymetrix platform

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Cheng

PROVIDER: E-GEOD-29137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Numb variant specific transcriptome analysis

Project description:Numb has 4 known variants and we are reporting two novel Numb variants, Numb5 and Numb6 in this study. The metastaticability variations between the numb variants were evaulated through the transcriptome.

2011-05-09 | GSE29137 | GEO

Effects on mRNA levels of a Numb and Numb-like conditional knockout in skeletal muscle fibers

Project description:We investigated the gene expression changes resulting from loss of Numb from skeletal muscle fibers using a conditional, inducible knockout of Numb and its close homolog Numb-like (NumbL) bulk RNA-sequencing.

2022-02-16 | GSE167390 | GEO

Comprehensive Identification of Phosphorylation Sites on the Numb Endocytic Adaptor Protein

Project description:Numb is an endocytic adaptor protein that functions in the endocytosis and intracellular trafficking of membrane receptors and adhesion molecules. Numb localization and function have been shown to be regulated by phosphorylation through atypcial protein kinase C at several key sites. Here, we use Liquid Chromatogrpahy, Tandem Mass Spectrometry to identify additional Numb phosphorylation sites not yet characterized.

2014-12-16 | PXD000997 | Pride

numb and numblike deleted by Nkx2.5Cre

Project description:The ventricular wall of the heart is composed of trabeculated and compactlayers, which are separated by yet unknown processes during embryonic development. Notch signaling plays important roles in cardiac development. Mutations in Notch signaling components are related to human congenital heart diseases Two homologues, Numb and Numblike (Numbl), are expressed in mammals, which also act as inhibitors of Notch signaling Here we investigate the role of Notch2 and Numb/Numblike during myocardial trabeculation and compaction. The aim of the experiment is to analyze whether Numb/Numblike are involved in inhibition of Notch activity in the developing heart.

2013-01-17 | E-MEXP-3490 | biostudies-arrayexpress

Regulation of NUMB alternative splicing by RBM5, RBM6 and RBM10 controls cancer cell proliferation

Project description:RBM5, a regulator of alternative splicing of apoptotic genes, and its close homologues, RBM6 and RBM10, are RNA binding proteins frequently deleted or mutated in lung cancer. We report that RBM5/6 and RBM10 antagonistically regulate the proliferative capacity of cancer cells and display distinct positional effects in alternative splicing regulation. We identify the Notch pathway regulator NUMB as a key target of these factors in the control of cell proliferation. NUMB alternative splicing, which is frequently altered in lung cancer, can regulate colony and xenograft tumor formation and its modulation recapitulates or antagonizes the effects of RBM5, 6 and 10 in cell colony formation. RBM10 mutations identified in lung cancer cells disrupt NUMB splicing regulation to promote cell growth. Our results reveal a key genetic circuit in the control of cancer cell proliferation.

2013-12-20 | GSE47431 | GEO