Multiple oxygen tension environments reveal diverse patterns of transcriptional regulation in primary astrocytes

ABSTRACT: The central nervous system normally functions at O2 levels which would be regarded as hypoxic by most other tissues. However, most in vitro studies of neurons and astrocytes are conducted under hyperoxic conditions without consideration of O2-dependent cellular adaptation. We analyzed the reactivity of astrocytes to 1, 4 and 9% O2 tensions compared to the cell culture standard of 20% O2, to investigate their ability to sense and translate this O2 information to transcriptional activity. Variance of ambient O2 tension for rat astrocytes resulted in profound changes in ribosomal activity, cytoskeletal and energy-regulatory mechanisms and cytokine-related signaling. Clustering of transcriptional regulation patterns revealed four distinct response pattern groups that directionally pivoted

ORGANISM(S): Rattus norvegicus

SUBMITTER: Kevin Becker 

PROVIDER: E-GEOD-29296 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress