Waves of early transcriptional activation and pluripotency program initiation along human preimplantation development.

ABSTRACT: The events regulating human preimplantation development are still largely unknown, due to scarcity of material, ethical and legal limitations, and lack of reliable techniques to faithfully amplify the transcriptome of a single cell. Nonetheless, knowledge in human embryology is gathering renewed interest due to its close relationship with both stem cell biology and epigenetic reprogramming to pluripotency, and their centrality to regenerative medicine. Using carefully timed genome-wide transcript analyses on single oocytes and embryos, the analysis of the data allowed us to uncover a series of successive waves of embryonic transcriptional initiation which start as early as the 2 cell stage. In addition, we identified hierarchical activation of genes involved in the regulation of pluripoten

ORGANISM(S): Homo sapiens

SUBMITTER: Stephanie Boue 

PROVIDER: E-GEOD-29397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress