Whole-transcriptome sequencing of the goat primary mammary epithelial cell line after challenge with Mycoplasma agalactiae

ABSTRACT: Whole-transcriptome sequencing of luminal epithelial cells was performed in order to profile the transcriptomic response to infection and to elucidate the immune response signaling capability of milk producing epithelial cells. Primary goat mammary epithelial cell (pgMEC) culture model, mimicking the function of luminal cells in vivo, was established and infected with Mycoplasma agalactiae (Ma). After infection total RNA was extracted from the cells at different time-points post infection (PI) and sequenced using Illumina Gene Analyzer IIx. Four datasets were generated including controls and pgMECs challenged for 3 h, 12 h, and 24 h. The obtained 50 bp sequences were mapped against the bovine reference transcriptome. In addition to analysing individual genes for differential expression (DE

ORGANISM(S): Capra hircus

SUBMITTER: Jakob Hedegaard 

PROVIDER: E-GEOD-30379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress