Comparative Genomics Reveals New Molecular targets for Accelerated Improvement of Erythromycin-producing Strains

ABSTRACT: Information about molecular mechanisms underlying improvement of antibiotic-producing microorganisms with traditional mutation and screening approach is largely missing. This information is essential to develop rational approaches to strain improvement. In this study by using comparative genomic analysis we have identified all genetic changes that have occurred during development of an erythromycin overproducer, which was obtained by the traditional mutate-and-screen method. Compared with parental Saccharopolyspora erythraea NRRL 2338, a total of 117 deletion, 78 insertion and 12 transposition sites were found across the genome of the overproducer. Among them, 71 insertion/deletion sites mapped within coding sequences (CDSs) generating frame-shift mutations. Moreover, single nucleotide var

ORGANISM(S): Saccharopolyspora erythraea

SUBMITTER: Clelia Peano 

PROVIDER: E-GEOD-30600 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress