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Profiling of Jurkat T cells activated with CD3, CD28 and PMA and multiple kinase inhibitors

ABSTRACT: T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into the Th1, Th2, Th17 or iTreg phenotype, depending on environmental co-stimulatory signals. In order to identify the genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed comprehensive transcriptome analyses of Jurkat T cells stimulated with various stimuli an pathway inhibitors Jurkat T cells were treated with CD3, CD28 and PMA and all pairwise combinations, in the presence of DMSO (control) or kinase inhibitors. RNA was isolated after 8 hrs incubation.

ORGANISM(S): Homo sapiens

SUBMITTER: Wynand Alkema

PROVIDER: E-GEOD-30674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profiling of Jurkat T cells activated with CD3, CD28 and PMA at 1 and 8 hours

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The polycomb protein Ezh2 regulates differentiation and plasticity of CD4 T helper type-1 and type-2 cells [ChIP-seq]

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