Proteomics

Dataset Information

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Human-derived PBMCs were stimulated by different groups to kill tumor cells.After 48 hours, PBMCs were taken out, and the enrichment of activated proteins of relevant lymphocytes and the regulation of pathways were examined by proteomics.

ABSTRACT: In this study, proteomics was used to explore the activation of lymphocytes and related pathways in PBMCs of different stimulation groups. In our study, proteomics analysis showed that the immune cells in PBMCs activated by stimulation group exhibit improved functions related to white blood cell activation, proliferation and cytotoxicity during biological processes. At the same time, KEGG pathway analysis showed that the signaling pathways’ key proteins for leukocyte transendothelial migration, Th1/Th2 cell differentiation, JAK-STAT (involved in many important biological processes such as cell proliferation, differentiation, apoptosis and immune regulation), DNA replication and Th17 cell differentiation were up-regulated; whereas the key proteins of necroptosis pathways in immune cells were down-regulated.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peripheral Blood Mononuclear Cell, Cell Culture

DISEASE(S): Melanoma

SUBMITTER: Jing Jin  

LAB HEAD: Binlei Liu

PROVIDER: PXD026739 | Pride | 2023-07-20

REPOSITORIES: Pride

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Publications

Bispecific Antibody Expressed by an Oncolytic Herpes Simplex Virus Type 2 Can Transform Heterologous T Cells Into Uniform Tumor Killer Cells.

Jin Jing J   Wang Runyang R   Yang Junhan J   Hu Han H   Wang Di D   Cai Linkang L   Fang Zhizheng Z   Dong Shuang S   Hu Sheng S   Wang Yang Y   Liu Binlei B  

Human gene therapy 20220601 11-12

BsAb (bispecific antibody)-armed oncolytic viruses (OVs) are effective in regulating tumor microenvironment. However, oHSV2 (oncolytic herpes simplex virus type 2) expressing immune checkpoints targeting BsAb molecules are not reported. Here, we generated oHSV2-armed PD-L1/CD3 BsAb and established pharmacodynamic evaluation models, which suggested that our oHSV2-BsAb molecules have an improved oncolytic potency <i>in vitro</i> and <i>in vivo.</i> The oHSV2 viruses armed with BsAb molecules targe  ...[more]

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