Global analysis of the Staphylococcus aureus response to mupirocin

ABSTRACT: In the present study we analyzed the response of S. aureus to mupirocin, the drug of choice for nasal decolonization of S. aureus. Mupirocin selectively inhibits the bacterial isoleucyl-tRNA synthetase (IleRSs) leading to the accumulation of uncharged isoleucyl-tRNA and hence (p)ppGpp. The latter is a signal for the induction of the stringent response, an important global transcriptional and translational control mechanism that allows bacteria to adapt to nutritional deprivation. To identify proteins with an altered synthesis pattern in response to mupirocin treatment we used the highly sensitive 2-dimensional gel electrophoresis technique in combination with mass spectrometry. Obtained results were complemented by DNA-microarray, Northern blot and metabolome analysis. Whereas expression o

ORGANISM(S): Staphylococcus aureus

SUBMITTER: FRANCOIS Patrice 

PROVIDER: E-GEOD-30743 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress