Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics

ABSTRACT: Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem cells present in murine bone marrow and human umbilical cord blood (hUCB) and as such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identity and properties of VSEL cells, particularly those derived from hUCB. For this reason, we have carried out an extensive characterisation of purified populations of VSEL cells from a large number of UCB samples. Consistent with a previous report, we find that VSEL cells are CXCR4+, have a high density, are indeed significantly smaller than HSC and have an extremely high nuclear/cytoplasmic ratio. Their nucleoplasm is unstructured and stains strongly with Hoechst 33342. A comprehensive FACS scree

ORGANISM(S): Homo sapiens

SUBMITTER: Andreas Heider 

PROVIDER: E-GEOD-31869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress