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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes.

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE27893: Genome-wide maps of chromatin state in early erythroid precursors versus later, more differentiated erythroblasts. GSE32110: RNA-seq expression profiles during terminal erythropoiesis Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Bill Wong

PROVIDER: E-GEOD-32111 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes.

Wong Piu P Hattangadi Shilpa M SM Cheng Albert W AW Frampton Garrett M GM Young Richard A RA Lodish Harvey F HF

Blood 20110822 16

It is unclear how epigenetic changes regulate the induction of erythroid-specific genes during terminal erythropoiesis. Here we use global mRNA sequencing (mRNA-seq) and chromatin immunoprecipitation coupled to high-throughput sequencing (CHIP-seq) to investigate the changes that occur in mRNA levels, RNA polymerase II (Pol II) occupancy, and multiple posttranslational histone modifications when erythroid progenitors differentiate into late erythroblasts. Among genes induced during this developm ...[more]

PMID: 21860024

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Genome-wide maps of chromatin state in early erythroid precursors versus later, more differentiated erythroblasts.

Project description:Here we globally analyzed mRNA and epigenetic changes in both early erythroid progenitors and late erythroblasts. Concomitant with gene induction there was an increase in RNA Pol II binding and activation marks near the transcriptional start site (TSS) and the elongation mark H3K79me2 (but not H3K36me3),both near the TSS and along the full gene length. In contrast, most repressed genes became depleted of elongation marks. We also found that relative changes in histone modification levels--in particular, H3K79me2 and H4K16ac-- most predictive of gene expression patterns. Our data suggests that in terminal erythropoiesis both transcriptional initiation and elongation contribute to induction of erythroid genes; in contrast, gene repression is mainly mediated by inhibition of Pol II elongation. Our data provides a comprehensive map of the epigenome during terminal erythroid differentiation. Examination of 7 different histone modifications and Pol II binding in 2 cell types.

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