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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Ezh2-null erythrocyte/megakaryocyte progenitor (MEP)

ABSTRACT: The polycomb group (PcG) proteins function in gene silencing through histone modifications. They form chromatin-associated multiprotein complexes, termed polycomb repressive complex (PRC) 1 and PRC2. These two complexes work in a coordinated manner in the maintenance of cellular memories through transcriptional repression of target genes. EZH2 is a catalytic component of PRC2 and trimethylates histone H3 at lysine 27 to transcriptionally repress the target genes. PcG proteins have been characterized as general regulators of stem cells, but recent works also unveiled their critical roles in cancer. Wild type and Ezh2 null MEP from fetal liver and adult bone marrow

ORGANISM(S): Mus musculus

SUBMITTER: ATSUSHI IWAMA

PROVIDER: E-GEOD-32929 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dependency on the polycomb gene Ezh2 distinguishes fetal from adult hematopoietic stem cells.

Mochizuki-Kashio Makiko M Mishima Yuta Y Miyagi Satoru S Negishi Masamitsu M Saraya Atsunori A Konuma Takaaki T Shinga Jun J Koseki Haruhiko H Iwama Atsushi A

Blood 20111031 25

Polycomb-group (PcG) proteins are essential regulators of hematopoietic stem cells (HSCs). In contrast to Bmi1, a component of Polycomb repressive complex 1 (PRC1), the role of PRC2 and its components in hematopoiesis remains elusive. Here we show that Ezh2, a core component of PRC2, is essential for fetal, but not adult, HSCs. Ezh2-deficient embryos died of anemia because of insufficient expansion of HSCs/progenitor cells and defective erythropoiesis in fetal liver. Deletion of Ezh2 in adult BM ...[more]

PMID: 22042701

Similar Datasets

Expression data from Ezh2-null erythrocyte/megakaryocyte progenitor (MEP)

Project description:The polycomb group (PcG) proteins function in gene silencing through histone modifications. They form chromatin-associated multiprotein complexes, termed polycomb repressive complex (PRC) 1 and PRC2. These two complexes work in a coordinated manner in the maintenance of cellular memories through transcriptional repression of target genes. EZH2 is a catalytic component of PRC2 and trimethylates histone H3 at lysine 27 to transcriptionally repress the target genes. PcG proteins have been characterized as general regulators of stem cells, but recent works also unveiled their critical roles in cancer.

2011-12-27 | GSE32929 | GEO

Expression data from Tet2-hypomorph (knockdown) and/or Ezh2-null Lineage-Sca-1+c-Kit+ (LSK) cells and granulocyte-macrophage progenitors (GMPs)

Project description:PcG proteins form the polycomb repressive complexes (PRC) 1 and 2, functioning as transcriptional repressors through histone modifications. They have been implicated in the maintenance of self-renewing somatic and cancer stem cells. PcG genes have been characterized as tumor suppressor genes as exemplified by somatic inactivating mutations of EZH2, a gene encoding histone methyltransferase in PRC2, in myeloid malignancy. Mice deficient for Tet2 have been reported to recapitulate some aspects of myeloid malignancies. We evaluated the Ezh2-deficient mice and also tested the impact of concurrent depletion of Ezh2 and Tet2 on hematopoiesis. Purified LSK cells and GMPs from BM of recipient mice repopulated with wild-type, Tet2KD/KD, Ezh2-/- and Tet2KD/KDEzh2-/- fetal liver cells were subjected to RNA extraction and hybridization on Agilent microarrays.

2013-08-01 | E-GEOD-42666 | biostudies-arrayexpress

Expression data from Ezh2-null leukemic cells

2012-05-31 | E-GEOD-33808 | biostudies-arrayexpress

Expression data from Ezh2-null leukemic cells

2012-06-01 | GSE33808 | GEO

The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation

2011-08-12 | GSE31354 | GEO

The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation

Project description:Polycomb group (PcG) proteins form multiprotein complexes, called Polycomb repressive complexes (PRCs). PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3). Suz12 is essential for PRC2 activity and its inactivation results in early lethality of mouse embryos. Total RNA was extracted independently from three WT and three Suz12 KO ES cell clones. RNA was quantified, and equal amounts fromthe three Suz12 WT and the three Suz12 KO samples were pooled into one sample to reduce the experimental variation. Targets for microarray hybridization were synthesized according to the supplier’s instructions (Affymetrix). Hybridization, washing, staining, scanning, and data analysis were performed at the Affymetrix microarray unit at the Institute of Molecular Oncology of the Italian Foundation for Cancer Research-European Institute of Oncology campus, Milan, Italy, according to the manufacturer’s instructions.

2011-08-11 | E-GEOD-31354 | biostudies-arrayexpress

Expression data from Tet2-hypomorph (knockdown) and/or Ezh2-null Lineage-Sca-1+c-Kit+ (LSK) cells and granulocyte-macrophage progenitors (GMPs)

2013-08-01 | GSE42666 | GEO

Chromatin immunoprecipitation of mouse polycomb group (PcG) proteins in embryonic stem cells

Project description:Polycomb group (PcG) proteins are highly conserved from flies to mammals and many of these factors have essential roles in early embryonic development. PcG proteins comprise two multimeric complexes, the Polycomb Repressive complexes 1 and 2 (PRC1 and 2), which have been shown to repress transcription through epigenetic modification of chromatin structure. To gain insight into the role of Polycomb in early development, we have identified PRC1 and PRC2 target genes in mouse embryonic stem (ES) cells using genome-scale location analysis. We found that PRC2 occupies many genes that encode key regulators of development including those encoding transcription factors and components of signaling pathways. These genes are repressed and contain nucleosomes methylated at lysine 27 on histone H3. The majority of PRC2 bound and methylated target genes are co-occupied by PRC1 indicating that these complexes function at a similar set of genes in ES cells. Lack of PRC1 or PRC2 subunits in ES cells results in derepression of target genes and loss of pluripotency. These results provide insight into how PcG proteins contribute to the maintenance of stem cell identity.

2006-05-09 | E-WMIT-12 | biostudies-arrayexpress

The dynamic interactome of Polycomb family complexes during stem cell differentiation

Project description:While the core subunits of Polycomb group (PcG) complexes are well characterized, little is known about the dynamics of these protein complexes during cellular differentiation. We used quantitative interaction proteomics to study PcG proteins in mouse embryonic stem cells (mESCs) and neural progenitor cells (NPCs). We found the stoichiometry of PRC1 and PRC2 to be highly dynamic during neural differentiation.

2016-05-19 | PXD003758 | Pride

proj022_chipseq

Project description:The mechanisms that recruit the major Polycomb-group (PcG) complexes PRC1 and PRC2 to target sites in vertebrate cells are not well understood. Building on recent studies that have determined a reciprocal relationship between DNA methylation and Polycomb activity, we demonstrate that in methylation deficient ES cells CpG density, combined with antagonistic effects of H3K9me3 and H3K36me3, redirect PcG complexes to pericentric heterochromatin and gene rich domains. In this experiment we have assayed the genomic distribution of H3K9me3 and H3K36me3 marks,

2014-05-23 | E-MTAB-2481 | biostudies-arrayexpress

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