Sexually Dimorphic Gene Expression in the Adult Mouse Hypothalamus and Bed Nucleus of the Stria Terminalis

ABSTRACT: To identify genes that are downstream of gonadal hormones and that control dimorphic behaviors, we used a MEEBO array platform to profile gene expression of adult male and female hypothalamus against a whole brain reference sample. The experimental design allowed us to identify genes that are upreguated in the hypothalamus compared to the whole brain and are dimorphically expressed between the two sexes. In situ hybridization of candiate genes were carried out to validate the dimorphic expression of these genes in the hypothalamus. Array results were used to create a list of genes to screen by in situ hybridization. For each normalization method, a list of genes that were upregulated in the male or the female was created, and a gene had to be on a set number of these individual normalizat

ORGANISM(S): Mus musculus

SUBMITTER: Xiaohong Xu 

PROVIDER: E-GEOD-33307 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress