Metabolic modeling of antibiotic and cytotoxic effects of the isoquinoline IQ-238 in S. aureus, S. epidermidis and human cells

ABSTRACT: Isoquinolines (IQs) are natural substances with antibiotic potential. IQ-238 is a synthetic analog of the novel-type N,C-coupled naphtylisoquinoline (NIQ) alkaloid ancisheynine. Gene expression data, cytotoxicity measurements and metabolic modelling is combined to assess the effects of the N,C-coupled naphtylisoquinoline (NIQ) compound IQ-238 on Staphylococcus aureus and man as a potential lead for novel antibiotics. It possesses a high activity against staphylococci but has low cytotoxicity in human cell lines. Genome annotation identified missed enzymes (validated by PCR) in the primary (e.g. nucleotide) metabolism of staphylococci. Gene expression changed after cultivation with IQ-238. Metabolic modelling did yield the adaptations of all central enzymes, including those not affected by

ORGANISM(S): Staphylococcus aureus

SUBMITTER: FRANCOIS Patrice 

PROVIDER: E-GEOD-33832 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress