Slx5_Slx8_Genome_Stability

ABSTRACT: The Saccharomyces cerevisiae Slx5/8 complex is the founding member of a recently defined class of ubiquitin ligases (STUbLs) that target sumoylated substrates. Slx5/8 has been implicated in genome stability and transcription, but the precise contribution to these processes is not clear. To characterize Slx5/8 function, we determined genome-wide changes in gene expression upon loss of either subunit. The majority of mRNA changes are part of a general stress response, also exhibited by mutants of other genome integrity pathways and therefore indicative of an indirect effect on transcription. Genome-wide binding analysis reveals a uniquely centromeric location for Slx5. Detailed phenotype analyses of slx5D and slx8D mutants show severe mitotic defects that include aneuploidy, spindle misposit

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Marian Groot Koerkamp 

PROVIDER: E-GEOD-33929 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress