Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin Accessibility Reveals Insight into Androgen Receptor Activation and Transcriptional Specificity

ABSTRACT: Using DNase-seq, mRNA-seq and publicly available ChIP-seq data sets, we examined the role of chromatin accessibility (DNase-seq) in androgen receptor binding to the genome (ChIP-seq) and AR-mediated transcriptional changes (mRNA-seq). Our data reveals genome-wide changes in chromatin structure that correspond to AR binding and differential gene expression. A focused examination of DNase-seq data around androgen receptor motifs within androgen receptor ChIP-seq peaks reveals distinct patterns of protection from DNaseI cleavage. Examination of chromatin accessibility (DNase-seq), AR binding (AR ChIP-seq), and transcription (mRNA-seq) in LNCaP cells before and after 12 hours of 1 nM R1881 treatment This Series represents the RNA-Seq data only. Exon microarray data generated under the same conditions is available through GSE15805. The DNase-seq data is publicly available through GSE32970 as well as the UCSC genome browser (genome.ucsc.edu) under Regulation::ENCODE DNase/FAIRE::Duke DNaseI HS:LNCaP and LNCaP + Andro. The accession numbers for the ChIP-seq experiments used are GSE14097 and GSE28126.

ORGANISM(S): Homo sapiens

SUBMITTER: Alok Tewari

PROVIDER: E-GEOD-34780 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Chromatin accessibility reveals insights into androgen receptor activation and transcriptional specificity.

Tewari Alok K AK Yardimci Galip Gürkan GG Shibata Yoichiro Y Sheffield Nathan C NC Song Lingyun L Taylor Barry S BS Georgiev Stoyan G SG Coetzee Gerhard A GA Ohler Uwe U Furey Terrence S TS Crawford Gregory E GE Febbo Phillip G PG

Genome biology 20121003 10

<h4>Background</h4>Epigenetic mechanisms such as chromatin accessibility impact transcription factor binding to DNA and transcriptional specificity. The androgen receptor (AR), a master regulator of the male phenotype and prostate cancer pathogenesis, acts primarily through ligand-activated transcription of target genes. Although several determinants of AR transcriptional specificity have been elucidated, our understanding of the interplay between chromatin accessibility and AR function remains ...[more]

PMID: 23034120

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Dataset Information

Chromatin Accessibility Reveals Insight into Androgen Receptor Activation and Transcriptional Specificity

Publications

Chromatin accessibility reveals insights into androgen receptor activation and transcriptional specificity.

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