The impact of Lactobacillus plantarum sortase on target-protein sorting, gastrointestinal persistence and host immunomodulation

ABSTRACT: Sortases are transpeptidase enzymes that couple surface proteins to the peptidoglycan of Gram-positive bacteria. Several sortase-dependent proteins (SDPs) have been identified that are crucial for bacterial pathogenesis and, although less frequently, for the physiology of non-pathogenic bacteria. We found that an isogenic sortase A (srtA) deletion derivative (NZ7104) of Lactobacillus plantarum WCFS1 did not express any residual SrtA activity, i.e. failed to cleave the LPQTDE SrtA recognition motif. Trypsination of intact bacterial cells, followed by mass spectrometry based peptide identification, revealed a significant decrease, but not complete loss of SDPs on the NZ7104 cell surface as compared to the wildtype. Using LiCl, we further found that several SDPs could be extracted from the ce

ORGANISM(S): Lactobacillus plantarum

SUBMITTER: Michiel Wels 

PROVIDER: E-GEOD-34999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress