Physiological and transcriptional response of Bacillus cereus treated with low-temperature nitrogen gas plasma

ABSTRACT: Aims: To investigate the inactivation kinetics of Bacillus cereus vegetative cells upon exposure to low-temperature nitrogen gas plasma and to reveal the mode of inactivation by transcriptome profiling. Methods and results: Exponentially growing B. cereus cells were filtered and put on agar plates. The plates, carrying the filters with the vegetative cells, were placed into nitrogen gas plasma at atmospheric pressure and cold temperature (~37°C). After different exposure times the cells were harvested for RNA extraction and enumeration. The RNA was used to perform whole transcriptome profiling using DNA microarrays. The transcriptome profile showed a large overlap with profiles obtained from conditions generating reactive oxygen species inside B. cereus. However, excess radicals such as

ORGANISM(S): Bacillus cereus

SUBMITTER: Maarten Mols 

PROVIDER: E-GEOD-35060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress