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Physiological and transcriptional response of Bacillus cereus treated with low-temperature nitrogen gas plasma

ABSTRACT: Aims: To investigate the inactivation kinetics of Bacillus cereus vegetative cells upon exposure to low-temperature nitrogen gas plasma and to reveal the mode of inactivation by transcriptome profiling. Methods and results: Exponentially growing B. cereus cells were filtered and put on agar plates. The plates, carrying the filters with the vegetative cells, were placed into nitrogen gas plasma at atmospheric pressure and cold temperature (~37°C). After different exposure times the cells were harvested for RNA extraction and enumeration. The RNA was used to perform whole transcriptome profiling using DNA microarrays. The transcriptome profile showed a large overlap with profiles obtained from conditions generating reactive oxygen species inside B. cereus. However, excess radicals such as peroxynitrite, hydroxyl and or superoxide were not found. Conclusions: Antibacterial activity of nitrogen gas plasmas is not based on UV radiation but on the formation of reactive oxygen or nitrogen species in the plasma jet rather than inside the targeted cells. Significance and impact of the study: This study represents the ﬁrst investigation of differential gene expression on a genome-wide scale in B. cereus following nitrogen gas plasma exposure. This study may help to design cheap, safe, and effective plasma decontamination devices.

ORGANISM(S): Bacillus cereus Bacillus cereus ATCC 14579

PROVIDER: GSE35060 | GEO | 2013/05/06

SECONDARY ACCESSION(S): PRJNA150933

REPOSITORIES: GEO

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Physiological and transcriptional response of Bacillus cereus treated with low-temperature nitrogen gas plasma

Project description:Aims: To investigate the inactivation kinetics of Bacillus cereus vegetative cells upon exposure to low-temperature nitrogen gas plasma and to reveal the mode of inactivation by transcriptome profiling. Methods and results: Exponentially growing B. cereus cells were filtered and put on agar plates. The plates, carrying the filters with the vegetative cells, were placed into nitrogen gas plasma at atmospheric pressure and cold temperature (~37°C). After different exposure times the cells were harvested for RNA extraction and enumeration. The RNA was used to perform whole transcriptome profiling using DNA microarrays. The transcriptome profile showed a large overlap with profiles obtained from conditions generating reactive oxygen species inside B. cereus. However, excess radicals such as peroxynitrite, hydroxyl and or superoxide were not found. Conclusions: Antibacterial activity of nitrogen gas plasmas is not based on UV radiation but on the formation of reactive oxygen or nitrogen species in the plasma jet rather than inside the targeted cells. Significance and impact of the study: This study represents the ﬁrst investigation of differential gene expression on a genome-wide scale in B. cereus following nitrogen gas plasma exposure. This study may help to design cheap, safe, and effective plasma decontamination devices. Plasma treated sampes compared with nitrogen gas flow treated samples and plasma treated samples compared to untreated control samples. Nitrogen flow samples in duplicate, plasma treated samples 4 replicates. Replicates hybridized with dyes swapped.

2013-05-06 | E-GEOD-35060 | biostudies-arrayexpress

Transcriptome comparison of S. pyogenes strains MGAS2221 and 2221ΔFasX following exposure to human plasma

Project description:The fasX gene encodes a small regulatory RNA in the group A Streptococcus (GAS). To determine the FasX regulon during GAS exposure to human plasma we compared parental strain MGAS2221 with isogenic fasX mutant strain 2221ΔFasX. Gene expression was analyzed 0, 15, and 60 minutes post-plasma exposure. GAS strains were grown in THY broth to mid-exponential phase, corresponding to an O.D.600 of 0.5. Two 16ml aliquots of each GAS culture were pelleted by centrifugation and the supernatant removed. Each GAS cell pellet was resuspended in 16ml of pre-warmed human plasma. Thus, four GAS-plasma suspensions were created, two each for each of the GAS strains, with one of the two using plasma from person A, and the other two using plasma from person B. Aliquots were recovered from each of the four GAS-plasma suspensions at T = 0, 15, and 60 mins. For the first two time-points 4.5ml of GAS-plasma suspension were added to 9ml of RNAprotect (Qiagen Inc.). For the final time-point, 4ml was added to 8ml of RNAprotect. Following addition of RNAprotect, samples were incubated at room temperature for 5 mins before centrifuging. Supernatant was removed and the pellets quick frozen in liquid nitrogen and stored at -80 °C until ready to use. RNA isolation, cDNA synthesis, and use of our custom Affymetrix microarray was as previously described (Perez et al., 2009)

2012-07-31 | E-GEOD-36645 | biostudies-arrayexpress

Bacillus cereus

Project description:Physiological and transcriptional response of Bacillus cereus treated with low-temperature nitrogen gas plasma

| PRJNA150933 | ENA

Transcriptome analysis of Bacillus subtilis in response to air plasma treatment

Project description:In this study, we present a follow up investigation on the effects of argon plasma on vegetative growing Bacillus subtilis 168 (see GSE27113). A growth chamber system suitable for low temperature gas plasma treatment of bacteria in liquid medium enabled a first glimpse on the complex cellular response. In order to gain further knowledge, a second kind of plasma treatment was applied and combined proteomic and transcriptomic analyses were used to investigate the specific stress response of B. subtilis cells to treatment with not only argon but also air plasma. Besides similar cellular responses, due to both argon and air plasma treatment, a variety of different responses such as growth retardation and morphological changes were observed. Furthermore, we analyzed the specific response to DNA damages and oxidative stress. The biological findings such as oxidative stress responses were supported by the detection of reactive plasma species by OES and FTIR measurements.

2013-08-22 | GSE32865 | GEO

Transcriptome analysis of Bacillus subtilis in response to air plasma treatment

2013-08-22 | E-GEOD-32865 | biostudies-arrayexpress

Transcriptome comparison of S. pyogenes strains MGAS2221 and 2221ΔFasX following exposure to human plasma

2012-08-01 | GSE36645 | GEO

Employment of exosome-mimetic nanovesicles as a plasma adjuvant for cancer targeting through Golgi stress-associated ferroptosis

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2024-05-03 | GSE231561 | GEO

Impact of gas plasma-induced chronic oxidative stress on head and neck cancer

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2023-01-19 | GSE223042 | GEO

Gas plasma combined with antibiotics for the eradication of methicillin-resistant Staphylococcus aureus biofilm

Project description:Gas plasma treatment caused polymerization of the SaClpP proteins and loss of protein function due to the oxidative modifications induced by reactive oxygen species of the gas plasma.

2021-09-09 | PXD023352 | Pride

Novel inducible filamentous sliding motility identified in pathogenic Bacillus cereus group species

Project description:We isolated an atmospheric contaminant, subsequently identified as a new strain of Bacillus mobilis, which showed a novel, robust, inducible filamentous sliding motility and completely colonized a bacterial culture plate in less than 48 h under some conditions. This flagella-independent sliding motility was characterized by long filamentous cells at the expanding edge, and was induced when cells were inoculated onto lawns of metabolically inactive Campylobacter jejuni cells, heat killed bacterial biomass, and milk or blood dried onto agar plates. Phosphatidylcholine (PC), bacterial membrane components, and sterile human fecal extracts were sufficient to induce filamentous expansion. Screening of eight other Bacillus spp. (five from the B. cereus group and three other Bacillus spp.) showed that filamentous motility was conserved amongst B. cereus group species to varying degrees. RNAseq of filamentously expanding cells collected from PC and milk lawn plates in comparison to rod-shaped cells from control plates revealed that genes related to metabolism, ion and amino acid transport were differently regulated, genes controlling sporulation were reduced, and some virulence genes (e.g., hblA/B/C/D and plcR) were increased. We hypothesize that the robust and conserved nature of filamentous motility in pathogenic B. cereus group species can enhance bacterial colonization during host colonization.

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