Transcriptional consequences of DISC1 knockdown in human neural progenitor cells

ABSTRACT: Background: One of the strongest identified risk factors for major psychiatric illness is a chromosomal translocation directly disrupting the DISC1 gene, which is observed in members of a large Scottish family. Although DISC1 is known to function through a variety of molecular pathways, no global assessment of the molecular consequences of cellular DISC1 perturbation has been carried out to date. Methods: Predicted effects of the t(1;11)(q42;q14) translocation on DISC1 expression were modelled in neural progenitor cells derived from human fetal cortex using RNA interference (RNAi). Downstream effects on the cellular transcriptome were assessed by microarray. The reproducibility of individual gene expression changes was tested in repeat experiments by quantitative PCR.Results: A total of 54

ORGANISM(S): Homo sapiens

SUBMITTER: Matthew Hill 

PROVIDER: E-GEOD-35548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress