The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria

ABSTRACT: Protein synthesis by ribosomes takes place on a linear substrate but at variable speeds. Transient pausing of ribosomes can impact a variety of co-translational processes, including protein targeting and folding. These pauses are influenced by the sequence of the mRNA. Thus redundancy in the genetic code allows the same protein to be translated at different rates. However, our knowledge of both the position and the mechanism of translational pausing in vivo is highly limited. Here we present a genome-wide analysis of translational pausing in bacteria using ribosome profiling-deep sequencing of ribosome-protected mRNA fragments. This approach enables high-resolution measurement of ribosome density profiles along most transcripts at unperturbed, endogenous expression levels. Unexpectedly, we

ORGANISM(S): Escherichia coli

SUBMITTER: Gene-Wei Li 

PROVIDER: E-GEOD-35641 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress