Degradome analysis in Phalaenopsis aphrodite subsp. formosana

ABSTRACT: We performed one degradome sequencing for identification and characterization of novel microRNAs in Phalaenopsis aphrodite subsp. formosana. Plant tissues of leaves, stalk, and flower buds frozen in liquid nitrogen were ground to fine powder using a mortar and pestle, respectively. Total RNA extraction was freshly prepared using Trizol (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions. About 50 μg of leaves with or without low temperature treatment, stalks and flower buds were taken separately and then mixed. The degradome library was constructed by the Genomics BioSci & Tech. Company following the manufactoring protocol. In brief, 200 μg of total RNA was passed though polyA column using the Oligotex kit (Qiagen). The 5'-RNA adapter containing a Mme I recognition

ORGANISM(S): Phalaenopsis aphrodite subsp. formosana

SUBMITTER: Feng-Ming An 

PROVIDER: E-GEOD-35739 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress