Promoter activity profiling throughout the Drosophila life cycle reveals role of transposons in regulatory innovation

ABSTRACT: Since their discovery, transposable elements have been proposed to play a central role in the evolution of their host genomes through their ability to regulate gene expression, in particular by providing transcription start sites (TSSs) for host genes. To investigate their contribution to developmental gene expression, we developed RAMPAGE, a high-throughput 5'-complete cDNA sequencing approach to accurately discover TSSs, characterize their transcripts, and quantify their expression. This strategy, which directly delineates the expression profiles of individual promoters and was designed to offer optimal sample multiplexing capabilities, represents an advantageous alternative to standard RNA-Seq for a wide range of transcriptome profiling applications. We used RAMPAGE in a genome-wide stu

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Philippe Batut 

PROVIDER: E-GEOD-36212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress