Transcriptome Analysis from non-alcoholic steatohepatitis (NASH)

ABSTRACT: The mechanisms underlying the progression of non-alcoholic steatohepatitis (NASH) are not completely elucidated. In this study we have integrated gene expression profiling of liver biopsies of NASH patients with translational studies in a mouse model of steatohepatitis and with pharmacological interventions in isolated hepatocytes to identify a novel mechanism implicated in the pathogenesis of NASH. By using high-density oligonucleotide microarray analysis we identified a significant enrichment of known genes involved in the multi-step catalysis of long chain polyunsaturated fatty acids, including delta-5 and 6 desaturases. A combined inhibitor of delta-5 and delta-6 desaturases significantly reduced intracellular lipid accumulation and inflammatory gene expression in isolated hepatocytes.

ORGANISM(S): Homo sapiens

SUBMITTER: Juanjo Lozano 

PROVIDER: E-GEOD-37031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress