Effects of DNA Methylation on Nucleosome Stability

ABSTRACT: Methylation of DNA at CpG dinucleotides represents one of the most important epigenetic mechanisms involved in the control of gene expression in vertebrate cells. In this report, we conducted high-throughput nucleosome reconstitution experiments on 572 KB of human DNA and 668 KB of mouse DNA that was unmethylated or methylated in order to investigate the effects of this epigenetic modification on the positioning and stability of nucleosomes. The DNA loci from both species contained the genes that encode the serum albumin family, the MYC protein, and the tumor suppressor p53. The results demonstrated that a small subset of nucleosomes positioned by nucleotide sequence was sensitive to methylation where the modification increased the affinity of these sequences for the histone octamer. Th

ORGANISM(S): Mus musculus

SUBMITTER: Clayton Collings 

PROVIDER: E-GEOD-37224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress