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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of S. cerevisiae where Histone H2B was mutated to give H2B^3-37 to identify genes regulated by the H2B N-terminal domain

ABSTRACT: Histone H2B was mutated to give H2B^3-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick

PROVIDER: E-GEOD-3804 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription.

Parra Michael A MA Kerr David D Fahy Deirdre D Pouchnik Derek J DJ Wyrick John J JJ

Molecular and cellular biology 20060501 10

Histone N-terminal domains are frequent targets of posttranslational modifications. Multiple acetylated lysine residues have been identified in the N-terminal domain of H2B (K6, K11, K16, K17, K21, and K22), but little is known about how these modifications regulate transcription. We systematically mutated the N-terminal domain of histone H2B, both at known sites of lysine acetylation and elsewhere, and characterized the resulting changes in genome-wide expression in each mutant strain. Our resu ...[more]

PMID: 16648479

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Project description:Histone H2B was mutated to give H2B K6,11,16,17,21,22G. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal acetylated lysine residues. Experiment Overall Design: mutant compared to wild-type, 3 replicates

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Project description:Histone H2B was mutated to give H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. This SuperSeries is composed of the SubSeries listed below.

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A Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT

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