Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein? regulates plant flowering by targeted alternative polyadenylation

ABSTRACT: Alternative polyadenylation (APA) is a widespread mechanism for gene regulation and has been implicated in flowering, but the molecular basis governing the choice of a specific poly(A) site during the vegetative-to-reproductive growth transition remains unclear. Here we characterize HLP1, an hnRNP A/B protein as a novel regulator for pre-mRNA 3’ end processing in Arabidopsis. Genetic analysis reveals that HLP1 suppresses Flowering Locus C (FLC), a key repressor of flowering in Arabidopsis. Genome-wide mapping of HLP1-RNA interactions indicates that HLP1 binds preferentially to A-rich and U-rich elements around cleavage and polyadenylation sites, implicating a role in 3’-end formation. We show significantly enriched binding of HLP1 to transcripts involved in RNA metabolism and flowering. Co

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: xiaofeng cao 

PROVIDER: E-GEOD-39051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress